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肾小球是肾脏器官滤过的基本功能单位,大多数肾脏疾病都是由于肾小球的硬化或纤维化而最终导致肾功能不全.分析肾小球的病理生理过程,必须对其生理功能有明确的认识,但由于研究手段有限,目前仍缺乏对正常肾小球的蛋白质成分全景式的分析.因此,本研究用蛋白质谱的方法,对正常大鼠的肾小球进行了全面的分析.在本研究中,取成年SD大鼠的肾脏组织,并分离出高纯度的肾小球.利用多维蛋白鉴定技术对大鼠肾小球进行全面系统的蛋白质组学分析.当假阳性率低于0.1%时,共鉴定出肽段19062个,其中非重复肽段11249个,归属于3796个蛋白,其中含有2个以上肽段2741个蛋白.我们对这些蛋白进行了功能注释和分类,发现了26个与已报道的肾小球(内皮细胞、足细胞和系膜细胞)Marker蛋白一致;利用HomoloGene将我们得到的大鼠肾小球蛋白质组学数据与其他文献报道的人和小鼠肾蛋白质组学数据进行了比较,为深入了解肾小球正常结构和功能提供重要的分子依据,为进行肾小球疾病的诊断和致病机制的研究奠定基础.
Glomerular filtration is the basic unit of kidney function, most of the kidney disease are due to glomerular sclerosis or fibrosis and eventually lead to renal insufficiency.Analysis of the pathophysiology of glomerular process, its physiological function must be However, due to limited research methods, there is still a lack of a panoramic analysis of the normal glomerular protein composition.Therefore, this study used the method of protein profiling to analyze the glomeruli of normal rats. In this study, we took the kidney tissue of adult SD rats and isolated the high-purity glomeruli.Using the multi-dimensional protein identification technique, we conducted a comprehensive and systematic proteomics analysis on rat glomeruli.When the false positive rate was lower than 0.162%, 19062 peptides were identified, of which 11249 were non-repetitive peptides, belonging to 3796 proteins, which contained 2741 proteins of more than two peptides.We annotated and classified these proteins functionally and found that 26 were consistent with reported glomerular (endothelial, podocyte, and mesangial) Marker proteins; we used rat homology between the rat glomerular proteomics data we obtained and other reported human and mouse Proteomics data were compared, it provides important insight into the molecular basis for the normal glomerular structure and function, provide a basis for diagnosis and pathogenesis of glomerular disease.