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OAW42细胞系是由一个卵巢乳头状浆液囊腺癌病人的腹水细胞建立的。腹水700g离心15分钟,细胞团再悬浮于Dulbe-cco改良的Eagle培养基中,补充以20%胎牛血清、20IU/ml胰岛素、1mM谷胺酰胺、1mM丙酮酸钠和3.7g/l碳酸氢钠。细胞每周传代一次,收获时用0.2μg/ml秋水仙碱和10μg/ml溴乙啶处理5小时后,用0.25%胰蛋白酶消化,细胞在培养40、68和88周后分别对21、36和52代细胞进行细胞遗传学分析。每次分析时计数50个分散良好的
The OAW42 cell line is established by ascites cells from a patient with ovarian papillary serous cystadenocarcinoma. The ascitic fluid was centrifuged at 700 g for 15 minutes and the cell pellet was resuspended in Dulbecco’s modified Eagle’s medium supplemented with 20% fetal bovine serum, 20 IU / ml insulin, 1 mM glutamine, 1 mM sodium pyruvate and 3.7 g / l bicarbonate sodium. The cells were passaged once a week, harvested with 0.2 μg / ml colchicine and 10 μg / ml ethidium bromide for 5 hours and then digested with 0.25% trypsin. Cells were cultured for 21, 36, And 52 generation cells for cytogenetic analysis. Count 50 well-dispersed for each analysis