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目的:本研究以RNA干扰技术下调PAR(prostate androgen regulated)基因表达,探讨其对前列腺癌PC3细胞增殖、凋亡及其相关蛋白Bcl-2/Bax表达水平的影响。方法:PC3细胞转染靶向PAR基因的siRNA,MTT法检测细胞增殖,用流式细胞术检测细胞凋亡,Western印迹检测Bcl-2和Bax蛋白表达水平。结果:PC3细胞PAR基因表达水平下调,此时处于G2-M期的细胞增加,为(29.95±3.25)%;细胞凋亡率亦明显增加,为(20.61±2.73)%,与对照组比较差异有显著性意义(P<0.01),同时Bcl-2蛋白表达下降,Bax表达水平升高,Bax/Bcl-2比值升高。结论:PAR基因表达下调可诱导细胞G2-M期阻滞和凋亡,其机制为抑制凋亡相关蛋白Bcl-2表达,同时促进Bax表达。
OBJECTIVE: To study the effects of RNA interference on the expression of prostate androgen regulated (PAR) genes in prostate cancer cell line PC3, and to explore their effects on the proliferation and apoptosis of PC3 cells and the expression of Bcl-2 / Bax protein. METHODS: PC3 cells were transfected with siRNA targeting PAR gene. Cell proliferation was detected by MTT assay. Apoptosis was detected by flow cytometry. Western blotting was used to detect the expression of Bcl-2 and Bax protein. Results: The expression of PAR gene in PC3 cells was down-regulated. The number of cells in G2-M phase was (29.95 ± 3.25)%, and the apoptosis rate was also significantly increased (20.61 ± 2.73)%, which was significantly lower than that in control group (P <0.01). At the same time, Bcl-2 protein expression decreased, Bax protein expression increased, Bax / Bcl-2 ratio increased. CONCLUSION: Down-regulation of PAR gene can induce cell G2-M arrest and apoptosis, and its mechanism is to inhibit Bcl-2 expression and promote Bax expression.