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卡瓦胡椒素B是药用植物卡瓦胡椒根中的一种天然查耳酮类化合物,研究了其对人非小细胞肺癌A549细胞的增殖抑制作用及其诱导细胞凋亡的分子机制.实验结果显示,卡瓦胡椒素B能显著抑制非小细胞肺癌A549细胞的增殖,且随着药物浓度的增加、处理时间的延长其抑制作用呈明显的剂量时间效应;同时,卡瓦胡椒素B能显著诱导A549细胞凋亡、细胞周期阻滞于G2-M期;分子机制研究表明,卡瓦胡椒素B能通过活化JNK激酶活性、下调凋亡抑制蛋白survivin的表达以及激活PARP活性从而导致其对A549细胞的增殖抑制作用.结果表明卡瓦胡椒素B对人非小细胞肺癌的预防与治疗可能具有潜在价值.
Kava pepper B is a natural chalcone compound in the root of medicinal plant Kava, and its inhibitory effect on proliferation of human non-small cell lung cancer A549 cells and its molecular mechanism of apoptosis induction were studied. The results showed that kavajapril B could significantly inhibit the proliferation of non-small cell lung cancer A549 cells, and with the increase of drug concentration, the inhibitory effect of prolonged treatment time showed a dose-time effect; at the same time, Significantly induced apoptosis of A549 cells and cell cycle arrest in G2-M phase. The molecular mechanism indicated that carbavalenol B could inhibit the activity of JNK kinase, down-regulate the expression of survivin and activate PARP A549 cell proliferation inhibitory effect.The results show that kava pepper B in the prevention and treatment of human non-small cell lung cancer may have potential value.