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单孢分离是植物病原真菌菌种纯化过程中一项不可缺少的技术,常见的有采用琼胶平板稀释纯化法或琼胶平板表面单孢子挑取法,近车又有人提出微块单孢子分离法。平板稀释纯化的可靠性较差,很难达到单孢子分离的目的;单孢子挑取可靠性虽高于前者,但不易掌握;微块法优于前两种方法,但获得单孢子的机率低,仅有10—20％。我们在菌种纯化的试验研究中,综合了目前较常用的几种方法,设计成印痕移取单孢子分离法。 一、操作器材的制备 (一)印痕器:由塑料套筒、胶塞、印痕筒3部分组成。 1.塑料套筒:根据所用显微镜低倍镜的外径大小,选择(可用试剂瓶的塑料内塞代
Single-spore isolation is an indispensable technology in the purification process of plant pathogenic fungi. Commonly, agar plate dilution purification method or agar plate surface single spore pick-up method is adopted. In addition, micro-spores single-spore separation method . Plate dilution purification is less reliable and difficult to achieve the purpose of single spore separation; single spore picking reliability is higher than the former, but not easy to grasp; micro-block method is superior to the first two methods, but the probability of obtaining single spores is low , Only 10-20%. In the experimental study on the purification of bacteria, we synthesized several commonly used methods currently and designed them as single-spore isolation method. First, the preparation of operating equipment (a) Marker: plastic sleeve, rubber stopper, impression cylinder 3 parts. 1. Plastic Sleeve: According to the diameter of the microscope used at low magnification, select (available plastic bottle of Cypriot