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目的:探讨肝癌组织中微小RNA-338-3p(miR-338-3p)的表达及与临床病理参数的关系。方法:选取2015年1月至2016年6月我院手术获得的67例肝癌组织标本,同时每例标本均取癌旁正常组织标本作为配对对照,采用实时定量逆转录聚合酶链反应(RT-qPCR)对两组组织标本中的miR-338-3p进行检测,并分析其与肝癌临床病理特征的关系。结果:45例(67.16%)miR-338-3p表达下调,22例(32.85%)表达上调;RT-qPCR结果显示,肝癌组织中miR-338-3p的相对含量为(0.76±0.38),低于癌旁正常组织中的(1.23±0.45),差异有统计学意义(t=-6.259,P=0.000)。miR-338-3p在低分化、TNM分期Ⅲ+Ⅳ期、肿瘤浸润深度T3+T4期、有淋巴结转移肝癌患者肝癌组织中的表达下调率高于中高分化、Ⅰ+Ⅱ期、T1+T2期、无淋巴结转移肝癌患者,差异有统计学意义(P<0.05)。不同性别、年龄、病理类型、肿瘤大小肝癌患者肝癌组织中miR-338-3p表达下调率差异无统计学意义(P>0.05)。结论:miR-338-3P在肝癌组织中呈低表达水平,与分化程度、TNM分期、肿瘤浸润深度、淋巴结转移有关,可能参与了肝癌的发生发展过程,早期检测可作为评估肝癌病情的指标。
Objective: To investigate the expression of microRNA-338-3p (miR-338-3p) in hepatocellular carcinoma and its relationship with clinicopathological parameters. Methods: A total of 67 HCC tissues obtained from our hospital from January 2015 to June 2016 were selected. Meanwhile, normal tissue adjacent tissues were taken as matched controls. Real-time quantitative reverse transcription-polymerase chain reaction (RT- qPCR) to detect the expression of miR-338-3p in two groups of tissues and analyze its relationship with clinicopathological features of hepatocellular carcinoma. Results: The expression of miR-338-3p was down-regulated in 45 cases (67.16%) and the expression of miR-338-3p in 22 cases (32.85%) was increased. The relative content of miR-338-3p in HCC was (0.76 ± 0.38) (1.23 ± 0.45) in the adjacent normal tissue, the difference was statistically significant (t = -6.259, P = 0.000). The downregulation of miR-338-3p in poorly differentiated, TNM stage Ⅲ + Ⅳ, tumor infiltration depth T3 + T4, and liver cancer with lymph node metastasis was higher than that in moderately well differentiated, stage Ⅰ + Ⅱ, stage T1 + T2 , No lymph node metastasis of liver cancer patients, the difference was statistically significant (P <0.05). There was no significant difference in down-regulation of miR-338-3p expression in HCC tissues of different gender, age, pathological type, tumor size and liver cancer (P> 0.05). Conclusion: The expression of miR-338-3P is low in HCC tissues. It may be involved in the development and progression of HCC with differentiation, TNM stage, depth of tumor invasion and lymph node metastasis. Early detection may be used as an index to evaluate the prognosis of HCC.