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以铁炮百合(Lilium longiforum)‘白天堂’的组培苗叶片为试材,通过RACE方法得到了一个热激转录因子(heat shock transcription factor,HSF)基因LlHSF1的cDNA全长序列。该序列全长1068bp,推断其含有一个780bp的开放阅读框(ORF),编码260个氨基酸,推导的蛋白质分子量为30.123kD。对推定的氨基酸序列与其它已知物种HSF进行比对发现,该基因有热激转录因子所具备的典型结构域和调控元件,据此推断,克隆到的基因是一个新的热激转录因子成员,命名为LlHSF1。荧光定量PCR分析结果表明:常温下该基因在百合根、鳞茎、叶中都有表达,但在叶片中表达量较高;实时荧光定量PCR分析结果表明,42℃处理1～12h,叶中该基因表达量增加。
The full-length sequence of LlHSF1, a heat shock transcription factor (HSF) gene, was obtained from tissue culture seedlings of Lilium longiforum ’White Heaven’ using RACE method. The full length of this sequence was 1068bp, which deduced that it contained a 780bp open reading frame (ORF) encoding 260 amino acids. The deduced protein had a molecular weight of 30.123kD. The deduced amino acid sequence was compared with HSF of other known species. The deduced amino acid sequence was found to have typical domains and regulatory elements of heat-shock transcription factor. Therefore, it was concluded that the cloned gene was a new heat shock transcription factor , Named LlHSF1. The result of real-time fluorescence quantitative PCR showed that the gene was expressed in roots, bulbs and leaves of lily at normal temperature but higher in leaves. The results of real-time fluorescence quantitative PCR showed that at 42 ℃ for 1 ~ 12 h, Gene expression increased.