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Phospholipase Dα(PLDα) is involved in plant response to salt stress,but the mechanisms remain unclear. We investigated rice PLDα(OsPLDa) localization and its effect on tonoplast(TP) and plasma membrane (PM) H~+ -ATPase activity and transcription in response to NaCI.When rice suspension-cultured cells were treated with 100 mM NaCI,PLDa activity in cell extracts showed a transient activation with a threefold increase at 1 h.The amount of OsPLDαprotein decreased slightly in the cytosolic fractions,whereas it increased significantly in the TP after NaCI treatment.OsPLDα1 knockdown cells were developed using RNA interference(RNAi) methods.The increase in TP and PM H -ATPase activity induced by NaCI was significantly inhibited in OsPLDα1-RNAi cells.Knockdown of OsPLDα1 prevented the NaCI-induced increase in the transcript level of OsVHA-A(encodes TP H~+-ATPase) and OSA2(encodes PM H~+-ATPase), as well as OsNHX1(encodes TP Na~+ /H~+ antiporter).The cells died more in OsPLDα1-RNAi mutant than in wild type when they were treated with NaCI.These results suggest that OsPLDαis involved in salt tolerance in rice through the mediation of H~+ -ATPase activity and transcription.
Phospholipase Dα (PLDα) is involved in plant response to salt stress, but the mechanisms remain unclear. We investigated rice PLDα (OsPLDa) localization and its effect on tonoplast (TP) and plasma membrane (PM) H ~ + -ATPase activity and transcription in response to NaCI.When rice suspension-cultured cells were treated with 100 mM NaCI, PLDa activity in cell extracts showed a transient activation with a threefold increase at 1 h. The amount of OsPLDαprotein decreased slightly in the cytosolic fractions, in the TP after NaCI treatment. OsPLDα1 knockdown cells were developed using RNA interference (RNAi) methods. increase in TP and PM H -ATPase activity induced by NaCI was identified inhibited in OsPLDα1-RNAi cells. Knockdown of OsPLDα1 prevented the NaCI-induced increase in the transcript level of OsVHA-A (encodes TP H ~ + -ATPase) and OSA2 (encodes PM H ~ + -ATPase), as well as OsNHX1 (encodes TP Na ~ + / H ~ + antiporter) more in OsPLDα1-RNAi muta nt than in wild type when they were treated with NaCI. These results suggest that OsPLDαis involved in salt tolerance in rice through the mediation of H ~ + -ATPase activity and transcription.