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目的 探讨ＣｏｎＡ对巨噬细胞（Ｍφ）活性的影响。方法以不同剂量的ＣｏｎＡ腹腔注射处理小鼠，４８ ｈ后收集腹腔Ｍφ做体外培养。分别以扫描及透射电镜观察Ｍφ的形态变化。分别以ＭＴＴ比色法和胸腺细胞增殖试验检测Ｍφ分泌ＴＮＦ－α和ＩＬ－１β的水平，并用免疫细胞化学染色法检测这两种蛋白的表达强度。结果ＣｏｎＡ作用后的Ｍφ，表现为体积增大、指状突起增加和胞浆内线粒体等细胞器增加。ＣｏｎＡ活化的腹腔Ｍφ分泌ＴＮＦ－α和ＩＬ－１β的水平增加，其在Ｍφ胞浆内的表达增强。结论ＣｏｎＡ具有激活Ｍφ增强其产生ＴＮＦ－α和ＩＬ－１β的作用。
Objective To investigate the effect of ConA on macrophage (Mφ) activity. Methods Mice were injected intraperitoneally with different doses of ConA. After 48 h, Mφ was collected and cultured in vitro. Morphological changes of Mφ were observed by scanning and transmission electron microscopy. The levels of TNF-α and IL-1β secreted by Mφ were detected by MTT colorimetric assay and thymocyte proliferation assay, respectively. The expression of these two proteins was detected by immunocytochemical staining. Results The Mφ after ConA treatment showed an increase in volume, an increase in finger-like protrusions and an increase in organelles such as mitochondria in the cytoplasm. ConA-activated peritoneal Mφ secreted increased levels of TNF-α and IL-1β, and enhanced its expression in Mφ cytoplasm. Conclusions ConA has the effect of activating Mφ to enhance its production of TNF-α and IL-1β.