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目的 :采用血清药理学方法观察芎芍胶囊对内皮素 (ET)致兔胸主动脉平滑肌细胞 (SMC)增殖凋亡的影响。方法 :分离正常兔胸主动脉SMC ,并传代培养 ,用ET造成胸主动脉SMC增殖模型。给正常兔灌胃芎芍胶囊混悬液 10天 ,制备含药血清 ,采用噻唑蓝 (MTT)比色法、流式细胞术及琼脂糖凝胶电泳法观察其对SMC增殖凋亡的影响。结果 :ET能明显促进SMC增殖 ,芎芍胶囊含药血清可减少S +G2 期SMC所占比例 ,对ET所致SMC增殖有明显抑制作用 ,并呈剂量依赖性 ,大剂量时尚可诱导SMC凋亡。结论 :芎芍胶囊可明显抑制ET所致SMC增殖 ,并有一定的诱导SMC凋亡的作用。这可能是其临床用于预防冠状动脉介入治疗后再狭窄的作用机制之
Objective : To observe the effect of Qiqi Capsule on proliferation and apoptosis of rabbit thoracic aorta smooth muscle cells (SMC) induced by endothelin (ET) by serum pharmacology. Methods: The SMCs of normal rabbit thoracic aorta were isolated and subcultured. The thoracic aorta SMC proliferation model was induced by ET. The normal rabbits were orally administered with capsule capsules for 10 days to prepare drug-containing serum. The effects of thiazolyl blue (MTT) colorimetry, flow cytometry, and agarose gel electrophoresis on proliferation and apoptosis of SMC were observed. RESULTS: ET could significantly promote the proliferation of SMC. The capsule contained serum could reduce the proportion of SMC in S + G2 phase and inhibit the proliferation of SMC induced by ET in a dose-dependent manner. Died. Conclusion :Ji Capsule can significantly inhibit the proliferation of SMC induced by ET and has a certain role in inducing SMC apoptosis. This may be the mechanism by which it is clinically used to prevent restenosis after coronary intervention