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AIM:To investigate the effect of cell adhesion molecule P-selectin monoclonal antibody (Mab) on metastasis andimmune function of mice orthototopically implanted withhuman gastric cancer tissue.METHODS:SCID mice were implanted orthotopically withSGC-7901 human gastric carcinoma tissue.Starting fromday 3 after operation,animals were given intravenously PBSor P-selectin Mab (100 μg/injection) (for both normal miceand tumor-implanted mice with tumors),twice weekly for 3weeks.Two animals in each group were sacrificed randomlyat the 1st,2nd,4th week and 6th week.While T cell and Bcell transformation indices were determined with the ~3H TdRinfiltration method,the NK cell activity was detected by theLDH release method.RESULTS:The metastatic rate in the P-selectin Mab treatedgroup was lower than that in the PBS treated group (withtumors).The NK activity of normal mice increased over time.The immune functions (T,B cell function,NK activity) of thetumor group in the 6th week were significantly lower thanthose in the 4th week,but the change was attenuated by P-selectin Mab.CONCLUSION:P-selectin Mab could suppress themetastasis of gastric cancer with no adverse effect on hostimmune function.
AIM: To investigate the effect of cell adhesion molecule P-selectin monoclonal antibody (Mab) on metastasis and immune function of mice orthototopically implanted with human gastric cancer tissue. METHODS: SCID mice were implanted orthotopically with SGC-7901 human gastric carcinoma tissue. Starting from day 3 after operation, animals were given intravenously PBSor P-selectin Mab (100 μg / injection) (for both normal mice and tumor-implanted mice with tumors), twice weekly for 3 weeks. Two animals in each group were sacrificed randomlyat the 1st, 2nd, 4th week and 6th week. Whilst T cell and Bcell transformation indices were determined with the ~ 3H TdRinfiltration method, the NK cell activity was detected by the LDH release method. RESULTS: The metastatic rate in the P-selectin Mab treated group was lower than that in the PBS treated group (withtumors). The NK activity of normal mice increased over time. Immune functions (T, B cell function, NK activity) of the tumor group in the 6th week were significantly l ower thanthose in the 4th week, but the change was attenuated by P-selectin Mab. CONCLUSION: P-selectin Mab could suppress themetastasis of gastric cancer with no adverse effect on hostimmune function.