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AIM:To investigate the expression of tumor suppressorgene p53 and spindle checkpoint gene Mad2,and todemonstrate their expression difference in colorectal cancerand normal mucosa and to evaluate its clinical significance.METHODS:Western blot and immunohistochemistry methodswere used to analyze the expression of Mad2 in colorectalcancer and its corresponding normal mucosa.The expressionof p53 was detected by immunohistochemistry method incolorectal cancer and its corresponding normal mucosa.RESULTS:Mad2 was significantly overexpressed in colorectalcancer compared with corresponding normal mucosa(P<0.001),and it was not related to the differentiation ofadenocarcinoma and other clinical factors(P>0.05).The ratioof Mad2 protein in cancer tissue(C)to that in its normalmucosa tissue(N)was higher than 2,which was morefrequently observed in patients with lymph gland metastasis(P<0.05).p53 protein expression was not observed in normalmucosa.The rate of p53 positive expression in adenocarcinomaswas 52.6 %.There was a significant difference betweenadenocarcinomas and normal mucosa(P<0.001),which wasnot related to the differentiation degree of adenocarcinomaand other clinical factors(P>0.05).CONCLUSION:Defect of spindle checkpoint gene Mad2and mutation of p53 gene are involved mainly in colorectalcarcinogenesis and C/N>2 is associated with prognosis ofcolorectal cancer.
AIM: To investigate the expression of tumor suppressor gene p53 and spindle checkpoint gene Mad2, and to demonstrate its expression difference in colorectal cancerand normal mucosa and to evaluate its clinical significance. METHODS: Western blot and immunohistochemistry methods used to analyze the expression of Mad2 in colorectal cancer and its corresponding normal mucosa. expression of p53 was detected by immunohistochemistry method incolorectal cancer and its corresponding normal mucosa. RESULTS: Mad2 was significantly overexpressed in colorectal cancer compared with corresponding normal mucosa (P <0.001), and it was not related to the differentiation of adenocarcinoma and The ratio of Mad2 protein in cancer tissue (C) to that in its normal mucosa tissue (N) was higher than 2, which was more frequently observed in patients with lymph gland metastasis (P <0.05) .p53 protein expression was not observed in normal mucosa. The rate of p53 positive expression in adenocarcinomas was was a significant difference between adenocarcinomas and normal mucosa (P <0.001), which was not related to the differentiation degree of adenocarcinoma and other clinical factors (P> 0.05). CONCLUSION: Defect of spindle checkpoint gene Mad2 and mutation of p53 gene are mainly involved in colorectal carcinogenesis and C / N> 2 is associated with prognosis of colorectal cancer.