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目的:研究不同生长周期的鼻咽癌低分化上皮细胞(CNE-2Z)的迁移能力及氯通道在迁移过程中所起的作用。方法:运用血清饥饿法、化学药物双阻断法、有丝分裂药物阻断及摇落法分别将CNE-2Z细胞同步化至细胞周期的G1、S、M期,流式细胞仪检测其同步化效果。结合应用迁移小室和图像分析法,测定迁移率。台盼蓝活细胞染色法测定药物的细胞毒性。结果:不同生长周期的细胞迁移能力不同,G1期细胞迁移能力最强,随后是M期,S期迁移能力最弱。氯通道阻断剂(ATP、NPPB、tamoxifen)抑制CNE-2Z细胞迁移,但不同的氯通道阻断剂对不同生长周期CNE-2Z细胞迁移的阻滞效应不相同。结论:CNE-2Z细胞的迁移能力与其所在的生长周期密切相关,氯通道在各期CNE-2Z细胞的迁移过程中起着重要作用。
Objective: To study the migration ability of nasopharyngeal carcinoma differentiated epithelial cells (CNE-2Z) with different growth cycles and the role of chloride channel in migration. Methods: CNE-2Z cells were synchronized to G1, S and M phases of cell cycle by serum starvation, double-block method of chemical drugs, blockage of mitotic drugs and shaking-off method, and the synchronization effect was detected by flow cytometry . Mobility was determined in conjunction with migration chamber and image analysis. Determination of Cytotoxicity of Drugs by Trypan Blue Living Cell Staining. Results: The cell migration ability in different growth cycle was different. The cell migration ability in G1 phase was strongest, followed by M phase, and the migration ability in S phase was the weakest. Chloride channel blockers (ATP, NPPB, tamoxifen) inhibited the migration of CNE-2Z cells, but the blocking effect of different chloride channel blockers on CNE-2Z cell migration in different growth cycles was different. CONCLUSION: The migration ability of CNE-2Z cells is closely related to the growth cycle of CNE-2Z cells. Chloride channel plays an important role in the migration of CNE-2Z cells.