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目的了解生猪受小肠结肠炎耶尔森菌的污染状况,为检验检疫工作和进行风险分析提供科学依据。方法利用方便抽样法在澳门市场抽取50个猪扁桃体样本,采用美国食品药物管理局微生物分析手册中的方法进行培养及生化鉴定,同时利用PCR方法对样本进行16S和ail基因检测。结果 50个猪扁桃体样本中,小肠结肠炎耶尔森菌的传统培养法的阳性率为12.0%(6/50),6个菌株的生物分型均为1A型,2个菌株为ail基因阳性;PCR检测方法的16S基因阳性率为36.0%(18/50),其中ail基因的阳性率为11.1%(2/18)。结论此次研究中,显示澳门生猪中存在小肠结肠炎耶尔森菌污染的风险,建议政府采取适当的预防和控制措施。此外,通过16S基因检测能缩短筛查的时间和提高检测效率,监测屠宰场或售卖市场的污染情况,保障食品安全。
Objective To understand the contamination status of pigs by Yersinia enterocolitica and provide a scientific basis for inspection and quarantine work and risk analysis. Methods 50 swine tonsils samples were collected from the Macau market by convenient sampling method. The samples were subjected to culture and biochemical identification by the method of Microbial Analysis Manual of the US Food and Drug Administration. Meanwhile, the 16S and ail genes were detected by PCR. Results The positive rate of Yersinia enterocolitica in 50 porcine tonsils samples was 12.0% (6/50), the genotypes of 6 strains were type 1A and the 2 strains were positive for ail gene The positive rate of 16S gene in PCR assay was 36.0% (18/50), of which the positive rate of ail gene was 11.1% (2/18). Conclusion The study shows the risk of Yersinia enterocolitica contamination in live pigs in Macao and suggests that the government should take appropriate preventive and control measures. In addition, the 16S gene test can shorten the screening time and improve the detection efficiency, monitor the slaughterhouse or the sale of the market pollution, to ensure food safety.