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目的建立以高效液相色谱-蒸发光散射检测法测定复方参芪软胶囊中黄芪甲苷含量的方法。方法色谱柱为AgilentHypersil ODS,流动相为乙腈-水(35∶65),流速为1.0 mL.min-1,进样量为20μL;蒸发光散射检测器条件:载气(压缩空气)压力为0.32 MPa,载气流量:2.6 L.min-1,漂移管温度为108℃。结果黄芪甲苷进样量的线性范围为0.402 4~4.024 0μg(r=0.999 8);平均回收率为100.02%(RSD=1.41%)。结论该方法准确度高、专属性强,可用于该制剂中黄芪甲苷的含量测定。
Objective To establish a method for the determination of Astragaloside Ⅳ in Fufang Shenqi Soft Capsules by high performance liquid chromatography with evaporative light scattering. Methods The column was Agilent Hypersil ODS. The mobile phase consisted of acetonitrile-water (35:65) and the flow rate was 1.0 mL.min-1. The injection volume was 20 μL. The conditions of evaporative light scattering detector were as follows: the pressure of carrier gas MPa, carrier gas flow rate: 2.6 L.min-1, drift tube temperature is 108 ℃. Results Astragaloside loading linear range of 0.402 4 ~ 4.024 0μg (r = 0.999 8); average recovery was 100.02% (RSD = 1.41%). Conclusion The method has high accuracy and specificity and can be used for the determination of astragaloside IV in the preparation.