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新鲜清洁的空气是保证动物生理机能和健康的必要条件。目前,国际上一般规定,无特定病原体(SPF)动物饲育室空气中细菌数应少于4个/平皿[按落菌法用普通琼脂平皿(φ9cm)暴露30分钟,37％培养48小时],相当于每立方米空气中细菌含量少于105个。清洁动物饲育室[即国外称的通常(Conven-tional)动物室]要求的新鲜空气,也须经一定程度的除菌、除尘和除臭,但尚无统一标准;按王楠田主译的《实验动物机构的建设》一书中指出,可参照人体容许值,一般认为细菌数应少于150个/平皿[方法同前,暴露时间为5分钟],即每立方米空气中细菌应少于23,595个。迄今,我国的大多数生物学实验研究,仍在使用未经微生物控制和环境控制的普通动物,提高实验动
Fresh and clean air is necessary to ensure the physiological function and health of animals. At present, the international community generally stipulates that the number of bacteria in the air of an animal-free room of a SPF-free animal should be less than 4 per plate [30 minutes for an average agar plate (φ9 cm) and 48 hours for a culture of 48 hours] The bacteria content is less than 105 per cubic meter of air. Fresh air required for clean animal shelters [that is, the so-called Conven-tional animal room abroad] also needs to be sterilized, dedusted and deodorized to a certain extent, but there is no uniform standard. According to “ The establishment of experimental animal institutions, ”a book that can refer to the human body allowable value is generally considered that the number of bacteria should be less than 150 / method [the same way as before, the exposure time of 5 minutes], that the bacteria per cubic meter of air should be less than 23,595. So far, most of the biological experimental research in our country is still using ordinary animals without microbial control and environmental control to improve experimental