论文部分内容阅读
作者把0.17×10~6个细胞/ml的Madin Darby牛肾细胞悬液植入含有5%胎牛血清单层MEM培养基内,在3%CO_2,37℃条件下培养。植入细胞数为1×10~6个细胞/培皿,细胞存活率用锥虫蓝检查达90%以上。细胞植入24小时后更换新鲜培养基。此时,在培养基中分别加入用磷酸缓冲液(PBS)配制的0.1～10.0μM不同浓度的氯化镉溶液,再培养24小时,检查贴壁细胞数,以此评价氯化镉对牛肾细胞的毒性。
The author put 0.17 × 10 ~ 6 cells / ml Madin Darby bovine kidney cell suspension in 5% fetal bovine serum containing MEM medium, cultured in 3% CO_2, 37 ℃ conditions. The number of implanted cells was 1 × 10 ~ 6 cells / culture dish, and the cell survival rate was over 90% with trypan blue. Fresh medium was changed 24 hours after cell implantation. At this time, different concentrations of cadmium chloride solutions of 0.1 to 10.0 μM formulated with phosphate buffered saline (PBS) were added to the culture medium, respectively, and cultured for a further 24 hours to examine the number of adherent cells, thereby evaluating the effect of cadmium chloride on bovine kidney Toxicity of cells.