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目的 :探讨载脂蛋白J(apoJ)基因在妊娠高血压综合征 (妊高征 )遗传背景中的地位。方法 :运用聚合酶链反应 变性梯度凝胶电泳 (PCR DGGE)技术对 5 0例妊高征患者和 5 0例正常孕妇的apoJ基因外显子进行了多态变异筛查。结果 :对apoJ基因 5号外显子筛查 ,共检出 38例杂合子和 1例纯合子 ,其中正常孕妇组有 2 5例杂合子和 1例纯合子 ;妊高征组有 1 3例杂合子 ,有 1例的 7号外显子也为杂合子。 5号外显子的杂合子多态变异基因型频率在妊高征组为 2 6 % ,显著低于对照组的 5 2 % (P <0 0 1 )。其余外显子DGGE未检出异常电泳条带。结论 :apoJ基因 5号外显子变异可能与妊高征的发病有关
Objective: To investigate the role of apoJ gene in the genetic background of pregnancy induced hypertension (PIH). Methods: Polymerase chain reaction denaturing gradient gel electrophoresis (PCR DGGE) was used to screen polymorphisms of apoJ exon in 50 PIH patients and 50 normal pregnant women. Results: ApoJ gene exon 5 screening, a total of 38 cases of heterozygotes and 1 case of homozygotes were detected, including normal pregnant women group of 25 cases of heterozygotes and 1 case of homozygous; PIH group of 13 cases of miscellaneous Zygotes, there are 1 cases of exon 7 is also heterozygous. The frequency of heterozygous polymorphism of exon 5 was 26% in PIH group, which was significantly lower than 52% in control group (P <0.01). The remaining exons DGGE did not detect abnormal electrophoresis bands. Conclusion: The variation of apoJ exon 5 may be related to the pathogenesis of PIH