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目的建立HPLC-ELSD法测定复方天麻蜜环糖肽片中黄芪甲苷的含量。方法色谱柱为Agilent C_(18)柱(250mm×4.6mm,5μm);流动相:乙腈-水(32∶68);流速:1.0ml·min~(-1);柱温:30℃;ELSD参数:漂移管温度为45℃;氮气吹扫流速为450kPa。采用外标二点法对数方程计算含量。结果黄芪甲苷进样质量浓度线性范围为0.0253~0.5060mg·ml~(-1),线性回归方程为log y=1.596log x+6.695,r=0.999(n=6);平均回收率为98.99%,RSD为1.87%(n=6)。结论该方法简便、准确、专属性强,可用于测定复方天麻蜜环糖肽片黄芪中黄芪甲苷的含量。
OBJECTIVE To establish a HPLC-ELSD method for the determination of astragaloside IV in compound Tianma honeycomb glycopeptide tablets. Methods The column was Agilent C 18 column (250 mm × 4.6 mm, 5 μm). The mobile phase was acetonitrile-water (32:68). The flow rate was 1.0 ml · min -1. Parameters: drift tube temperature 45 ° C; nitrogen purge flow rate 450kPa. Using external standard two-point logarithmic equation to calculate the content. Results The linear regression equation was log y = 1.596 log x + 6.695, r = 0.999 (n = 6), the average recovery was 98.99. The linear range was 0.0253 ~ 0.5060 mg · ml -1 %, RSD 1.87% (n = 6). Conclusion The method is simple, accurate and specific and can be used to determine the content of astragaloside Ⅳ in Radix Astragali.