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目的探讨疟疾感染早期根治性治疗对再感染细胞免疫应答的影响。方法用伯氏疟原虫感染DBA/2小鼠,感染后3d进行根治性治疗,并于初次感染后90d进行再感染。通过吉姆萨薄血膜染色法计数红细胞感染率,流式细胞术检测再感染前(0d)和再感染后(1、3、5d)不同时间点脾T细胞中活化性T细胞百分含量,ELISA检测脾细胞培养上清中IFN-γ、TNF-α、IL-4和IL-10水平。结果同源疟原虫再感染后,根治性治疗小鼠仅出现短暂的低水平虫体血症;再感染后第1～5天活化性T细胞百分率持续升高。IFN-γ于再感染后第1天即出现有意义的升高,第3天达到峰值水平,与此同时,TNF-α和IL-10水平也开始出现有意义的升高,但IL-4的升高出现在再感染后的第5天。结论疟疾感染早期的根治性治疗并不影响宿主在再感染时产生有效的细胞免疫应答,CD4+Th1应答反应也是抵御疟疾再感染的关键因素之一。
Objective To investigate the effect of early radical treatment of malaria infection on re-infected cellular immune response. Methods DBA / 2 mice were infected with Plasmodium berghei. After 3 days of infection, radical treatment was performed and re-infected 90 days after the initial infection. The infection rate of erythrocytes was counted by Giemsa thin membrane staining. The percentage of activated T cells in splenic T cells at different time points before re-infection (0d) and after re-infection (1,3,5d) were detected by flow cytometry. The levels of IFN-γ, TNF-α, IL-4 and IL-10 in the culture supernatant of spleen cells were detected by ELISA. Results After re-infection with Plasmodium falciparum, there was only a brief transient low level of parasitemia in mice treated with the same challenge. The percentage of activated T cells in the first to fifth days after re-infection continued to increase. IFN- [gamma] increased significantly on day 1 after re-infection and peaked on day 3. At the same time, TNF- [alpha] and IL-10 levels also started to show significant increases, but IL-4 The increase occurred on the 5th day after reinfection. Conclusion The early radical treatment of malaria infection does not affect the host to produce an effective cellular immune response when re-infected, CD4 + Th1 response is also one of the key factors to prevent re-infection of malaria.