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目的验证血清中抗环瓜氨酸抗体(Anti-cycliccitrulinated peptid,Anti-CCP)检测对类风湿关节炎(Rheumatoid Arthritis,RA)的临床诊断价值。方法选取2012—2014年某医院经检测诊断为RA病人68例作为RA组,非RA的自身免疫性疾病的81例患者作为非RA组,同时选取健康体检者90例作为对照。采集外周血4.0ml,分离血清,应用酶联免疫吸附试验(ELISA)检测抗-CCP抗体,将检测结果进行对比分析。结果 RA组血清中Anti-CCP的阳性率为92.65%,明显高于非RA组的16.05%及正常对照组的2.22%,经过率的标准化后,这种差异有显著性(P<0.01)。结论 RA患者血清中Anti-CCP的灵敏度、特异度明显高于非RA组及正常对照组;血清Anti-CCP检测可以作为RA的临床检测指标。
Objective To verify the clinical value of anti-cyclic citrullinated peptid (Anti-CCP) in the diagnosis of rheumatoid arthritis (RA). Methods Totally 81 patients diagnosed as RA patients and 68 non-RA autoimmune diseases in a hospital diagnosed as RA from 2012 to 2014 were selected as non-RA group and 90 healthy control subjects were selected as control. Blood samples were collected from 4.0 ml of peripheral blood and serum was separated. Anti-CCP antibody was detected by enzyme linked immunosorbent assay (ELISA), and the results were compared. Results The positive rate of serum anti-CCP in RA group was 92.65%, which was significantly higher than 16.05% in non-RA group and 2.22% in normal control group. The difference was significant (P <0.01) after standardized rate. Conclusion The sensitivity and specificity of anti-CCP in RA patients were significantly higher than those in non-RA patients and normal controls. Serum Anti-CCP test could be used as a clinical indicator of RA.