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目的:探讨不同干预手段,对骨折大鼠愈合过程中骨折部位CD34、FⅧ-RAg及血管再生通路VEGF/VEGFR-2的影响,为加速骨折愈合提供理论依据。方法:选取80只3月龄SD大鼠,随机分为4组,分别为骨折模型组;骨折+动员剂组;骨折+被动收缩组;骨折+动员剂+被动收缩组。骨折后分别在2、4、6、8周取材,制成脱钙骨切片;免疫组织化学染色方法测试骨折愈合过程骨痂处CD34、FⅧ-RAg及血管再生通路VEGF/VEGFR-2表达。结果:免疫组化结果显示,各干预手段均可增加CD34、FⅧ-RAg、VEGF、VEGFR-2在大鼠骨折部位的表达量。且动员剂+被动收缩组>被动收缩组>动员剂组>模型组。结论:骨骼肌被动收缩和(或)rhG-CSF干预均可促进造血干细胞/内皮祖细胞(HSCs/EPCs)的动员,促进血管再生通路VEGF/VEGFR-2的表达,促进血管再生,且被动收缩协同rhG-CSF动员的双重作用效果更为显著。此方法简单、易行,为骨折病人早期运动康复手段与方法的筛选提供基础理论依据。
OBJECTIVE: To investigate the effects of different interventions on the expression of CD34, FⅧ-RAg and VEGF / VEGFR-2 in the fracture site during fracture healing in rats, which may provide a theoretical basis for accelerated fracture healing. Methods: Eighty 3-month-old SD rats were randomly divided into 4 groups: fracture model group, fracture + mobilizer group, fracture + passive contraction group, fracture + mobilizer + passive contraction group. The fractured calluses were harvested at 2, 4, 6, and 8 weeks respectively to make decalcified bone slices. The expressions of CD34, FⅧ-RAg and VEGF / VEGFR-2 were detected by immunohistochemical staining. Results: The results of immunohistochemistry showed that all interventions could increase the expression of CD34, FⅧ-RAg, VEGF and VEGFR-2 in the fracture site of rats. And mobilization + passive contraction group> passive contraction group> mobilization group> model group. CONCLUSIONS: Skeletal muscle passive contraction and / or rhG-CSF intervention can promote the mobilization of hematopoietic stem / progenitor cells (HSCs / EPCs), promote the expression of VEGF / VEGFR-2, promote angiogenesis and passive contraction The synergistic effect of rhG-CSF mobilization is more pronounced. The method is simple and easy to operate and provides the basic theoretical basis for the screening of means and methods of early exercise rehabilitation for fracture patients.