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为建立对虾无包涵体杆状病毒的快速诊断技术，应用PCR技术分别对暴发性流行病发生前、流行中和发病后的对虾样品进行了检测．根据已克隆的对虾杆状病毒部分基因组序列而设计的引物能特异性地扩增出靶DNA片段，最低可以检测1pg的病素DNA．由典型发病症状对虾的胃、腮、肝胰腺、中肠、游泳足、肌内和心脏等器官和组织中成功地检测到病毒．不同组织和器官经扩增得到的信号强弱不同：病虾的胃扩增得到的信号最强，中肠、肌肉、心脏和游泳足次之，腮和肝胰腺信号最弱，说明病毒在不同组织和器官中数量及感染程度不同．在对虾发病前及发病后，用二次PCR还能检测表面不发病呈隐性感染状态的对虾携带的病毒；而对野生健康虾的检测结果为阴性．研究表明，PCR是检测对虾暴发性流行病快速、灵敏而准确的方法，对病毒病的早期诊断、防治和高健康对虾品种的选育具有指导意义．
In order to establish a rapid diagnostic technique for inclusion bodies of shrimp without inclusion bodies, the PCR technique was applied to detect the samples of prawn before, during and after the epidemic. Primers designed on the basis of the partial genome sequence of the cloned shrimp baculovirus can specifically amplify target DNA fragments with a detection limit of 1 pg of the pathogenic DNA. Viruses were successfully detected in organs and tissues of the stomach, gills, hepatopancreas, midgut, swimming foot, intramuscular and cardiac organs of the shrimp by the typical symptom of the disease. Different tissues and organs amplified signal strength is different: the shrimp of the stomach amplified the strongest signal, the midgut, muscle, heart and swimming foot times, gills and hepatopancreas signal is the weakest, indicating that the virus in different The quantity and level of infection in tissues and organs varies. Before and after the onset of shrimp, secondary PCR was also able to detect the virus carried by shrimp infected with the recessive virus on the surface of the shrimp; however, the test results of wild shrimp were negative. Studies have shown that PCR is a rapid, sensitive and accurate method for the detection of epidemic shrimp epidemic. It is instructive for the early diagnosis, prevention and treatment of viral diseases and breeding of healthy shrimp breeds.