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应用免疫组化S－P法，研究47例原发性肺癌手术标本中nm23－H1，PCNA，CD15的表达及其与区域性淋巴结转移的关系。结果显示：在原发性肺癌中nm23－H1，PC－NA，CD15的阳性表达分别为51．06％，65．96％，51．06％。其中nm23－H1在鳞癌中阳性表达43．3％（13／30），腺癌64．71％（11／17），腺瘤表达较鳞癌高。在鳞癌中无肺门及纵隔淋巴结转移者阳性62．50％（10／16），有转移者阳性21．41％（3／14）（P＜0．01），显示nm23－H1表达与鳞癌的肺门及纵隔淋巴结转移呈负相关。在PCNA表达中，有区域淋巴结转移的阳性表达为82．6％（19／23），无淋巴结转移为54．16（13／24）（P＜0．01），低分化癌阳性表达88．8％（8／9），明显高于高分化癌43．75％（7／16），（P＜0．01）。在CD15表达中，有淋巴结转移组中阳性表达为78．26％（18／23），无区域性淋巴结转移组中阳性表达25％（P＜0．01）。而nm23-H1，PCNA，CD15表达之间无显著相关性。本研究提示nm23－H1，PCNA，CD15的表达对肺癌预后评估有重要意义。
Immunohistochemical S-P method was used to study the expression of nm23-H1, PCNA and CD15 in 47 specimens of primary lung cancer and its relationship with regional lymph node metastasis. The results showed that the positive expression of nm23-H1, PC-NA and CD15 in primary lung cancer were 51.06%, 65.96% and 51.06%, respectively. Among them, nm23-H1 was positively expressed in squamous cell carcinoma by 43.3% (13/30) and adenocarcinoma by 64.71% (11/17). The expression of adenoma was higher than that of squamous cell carcinoma. No hilar and mediastinal lymph node metastases were positive in 62.50% (10/16) of the squamous cell carcinomas and 21.41% (3/14) were positive in the metastasis (P<0.01), indicating the expression of nm23-H1 The hilar and septal lymph node metastases of squamous cell carcinoma were negatively correlated. In the expression of PCNA, the positive expression of regional lymph node metastasis was 82.6% (19/23), no lymph node metastasis was 54.16 (13/24) (P<0.01), and poorly differentiated cancer was positively expressed. 8% (8/9) was significantly higher than that of well-differentiated carcinoma 43.75% (7/16) (P<0.01). In CD15 expression, the positive expression was 78.26% (18/23) in the lymph node metastasis group and 25% in the no regional lymph node metastasis group (P<0.01). There was no significant correlation between the expression of nm23-H1, PCNA and CD15. This study suggests that the expression of nm23-H1, PCNA and CD15 is of great significance in the evaluation of prognosis of lung cancer.