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为探讨ＨＢｓＡｇ阴性甚至ＨＢＶ标志（ＨＢＶＭ）全部阴性血清中检出ＨＤＶ标志（ＨＤＶＭ）的可能性及原因，本文对４６５例病毒性肝炎患者进行了血清ＨＤＶ综合标志的检测，于ＨＢｓＡｇ阳性及阴性血清中均可检出ＨＤＶ标志，分别为２６．６２％（７０／２６３）和２３．３９％（２９／１２４）．不仅如此，甚至于ＨＢＶＭ全部阴性患者中亦可检出ＨＤＶＭ２１．７９％（１７／７８）。用聚合酶链反应（ＰＣＲ）法检测发生ＨＤＶ感染的ＨＢｓＡｇ阴性或ＨＢＶＭ阴性血清中的ＨＢＶＤＮＡ，结果发现７５％（１５／２０）为阳性，提示上述阴性血清中多数存在ＨＢＶ体内复制及感染性，因检测技术及水平所限而未测出ＨＢＶＭ。同时对上述阴性血清随访６～２１个月，得出于ＨＢｓＡｇ阴性或ＨＢＶＭ阴性血清中检出ＨＤＶＭ的其它５种可能的原因。
In order to investigate the possibility and cause of detecting HDV marker (HDVM) in all HBsAg-negative and even HBVM negative serums, 465 viral hepatitis patients were tested for serum HDV comprehensive markers. HBsAg positive and negative serum HDV markers were detected in 26.62% (70/263) and 23.39% (29/124) respectively. Moreover, HDVM was detected in 21.79% (17/78) of all negative HBVM patients. HBVDNA in HBsAg-negative or HBVM-negative sera with HDV infection was detected by polymerase chain reaction (PCR), and found that 75% (15/20) were positive, suggesting that most of the negative sera contained in vivo HBV replication and infectivity, Due to the detection technology and the level of limited but not detected HBVM. At the same time, the above-mentioned negative serum was followed up for 6 to 21 months, and other 5 possible causes of HDVM detected in HBsAg-negative or HBVM-negative sera were obtained.