论文部分内容阅读
本研究采用小牛垂体提取液199培养基及普通199培养基分别培养人胃粘膜细胞，在培养期间不同时间测定DNA合成（3H－TDR渗入法），培养液PH（产酸），胃蛋白酶。结果表明，部分细胞贴壁生长，多数呈悬浮生长，细胞呈多角形式梭形，可见腺体样结构，分泌大量粘液。垂体培养基可维持培养24天，普通培养基16天，且增殖速度前者较快。3H－TDR渗入法表明垂体培养基培养的细胞cpm值显著高于普通培养基（P＜0.05）；而且产改、胃蛋白酶亦明显增多（P＜0．01和P＜0.05）。王者基本相互吻合。综合结果提示，小牛垂体提取液有促进体外胃粘膜细胞DNA的合成、增殖、产酸、产胃蛋白酶的作用。为研究胃粘膜细胞生理、病理变化及癌变提供了一个体外模型的新途径。
In this study, human gastric mucosal cells were cultured in 199 medium of calf pituitary extract and normal 199 medium. DNA synthesis (3H-TDR infiltration method), pH (acidogenicity) and pepsin of culture medium were measured at different time during culture. The results showed that some cells adherent growth, the majority of suspended growth, the cells were fusiform, showing gland-like structure, secretion of a large number of mucus. Pituitary medium can be maintained for 24 days, normal medium for 16 days, and the proliferation rate of the former faster. 3H-TDR infiltration method showed that the cpm value of cells cultured in pituitary culture was significantly higher than that in normal medium (P <0.05), and pepsin was significantly increased (P <0.01 and P <0.05). King basic mutual agreement. The comprehensive results suggest that the calf pituitary extract can promote gastric mucosal cells in vitro DNA synthesis, proliferation, acid production, the role of pepsin. It provides a new way to study the in vitro model of gastric mucosal cell physiology, pathological changes and carcinogenesis.