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目前国内多采用化学法和化学发光法来检测超氧化物岐化酶(SOD)的活力,而直接应用SOD单克隆抗体(McAb)ELISA双抗夹心法来检测其活性单位的报道还甚少。本文应用细胞杂交瘤技术,成功制备出了抗SODMcAb,并对其免疫学特性进行了分析,现介绍如下: 1 材料与方法 1.1 SOD:购自北京化学试剂公司。 1.2 人SOD、牛SOD、羊SOD:本室自制。取人、牛、羊的红细胞,分别用生理盐水洗四次,加等量蒸馏水使其溶血,缓慢加入0.4体积的-30℃三氯甲烷/乙醇液(3:5混合),再加入0.1体积蒸馏水,离心,收
At present, the activity of superoxide dismutase (SOD) is mostly detected by chemical and chemiluminescence methods. However, there are few reports on the detection of active units by the direct use of SOD monoclonal antibody (McAb) ELISA sandwich ELISA. In this paper, the use of cell hybridoma technology, successfully prepared anti-SODMcAb, and its immunological properties were analyzed are as follows: 1 Materials and methods 1.1 SOD: purchased from Beijing Chemical Reagent Company. 1.2 human SOD, cattle SOD, sheep SOD: homemade room. Take red blood cells of human, bovine and sheep, respectively, washed four times with saline, plus an equal amount of distilled water to hemolysis, slowly added 0.4 volume of -30 ° C trichloromethane / ethanol solution (3: 5 mixture) Distilled water, centrifuged, closed