论文部分内容阅读
目的研究1,4-苯醌(1,4-benzoquinone,BQ)对中国仓鼠肺成纤维细胞(V79)的毒性作用及DNA损伤效应,探讨BQ遗传毒作用机制及毒作用剂量范围。方法应用噻唑蓝(MTT)比色法检测不同浓度BQ作用不同时间对V79细胞的增殖抑制作用;单细胞凝胶电泳技术(SCGE)检测不同浓度BQ处理V79细胞后DNA损伤的差异。结果在2 h处理过程中,当BQ终浓度在0.025~0.05 mmol/L范围内,对细胞增殖的抑制作用不明显。当浓度≥0.1 mmol/L时,各染毒组吸光值下降,与阴性对照组比较,差异有统计学意义(P<0.05),并存在剂量?反应和时间?反应关系。SCGE结果显示,在0.0125~0.10 mmol/L BQ作用后,彗星细胞拖尾率随着浓度的增加而增加,且差异有统计学意义(P<0.05)。而彗星尾长、Olive尾矩和彗尾DAN%明显高于阴性对照组,差异有统计学意义(P<0.05),且随着BQ浓度的增加,损伤呈上升趋势。结论BQ能明显抑制V79细胞的增值,并在较低浓度下可以引起DNA单链断裂,说明遗传物质的损伤发生在细胞损伤早期。
Objective To investigate the toxic effects and DNA damage effects of 1,4-benzoquinone (BQ) on Chinese hamster lung fibroblasts (V79) and explore the mechanism of BQ genetic toxicity and the dose range of toxic effects. Methods MTT assay was used to detect the proliferation of V79 cells treated with different concentrations of BQ for different periods of time. Single cell gel electrophoresis (SCGE) was used to detect the DNA damage in V79 cells treated with different concentrations of BQ. Results In the 2 h treatment, when BQ was in the range of 0.025 ~ 0.05 mmol / L, the inhibitory effect on cell proliferation was not obvious. When the concentration was above 0.1 mmol / L, the absorbance of each exposure group decreased, compared with the negative control group, the difference was statistically significant (P <0.05), and there was dose-response and time-response relationship. The results of SCGE showed that the tailing rate of comet cells increased with the concentration of 0.0125 ~ 0.10 mmol / L BQ, and the difference was statistically significant (P <0.05). The tail length, Olive tail moment and tail DNA% of tail were significantly higher than that of the negative control group (P <0.05). The damage increased with the increase of BQ concentration. Conclusion BQ can significantly inhibit the proliferation of V79 cells and can cause DNA single strand breaks at lower concentrations, indicating that genetic damage occurs in the early stage of cell injury.