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为研究不同形态氮素(NH+4-N和NO-3-N)和水分胁迫互作时水稻渗透调节的差异,利用营养液培养,通过PEG(6000)对局部根系进行水分胁迫,分析比较水稻各部位主要渗透调节物质含量的变化。结果表明:局部根系水分胁迫时,供NH+4-N时受胁迫一侧根系生长无明显变化,根系可溶性糖和游离氨基酸含量分别增加64.5%和167%,根系木质部和韧皮部汁液中的可溶性糖含量分别增加243%和42.2%;而供NO-3-N时受胁迫一侧根系韧皮部汁液中可溶性糖含量显著降低,根系生长受到明显抑制,尽管可溶性糖和游离氨基酸含量分别增加46.4%和73.9%,韧皮部汁液中可溶性糖含量显著降低。未受胁迫一侧根系生物量累积则明显增大,这与其韧皮部汁液中的可溶性糖含量明显升高相一致。NH+4-N营养使水稻通过渗透调节有效抵抗水分胁迫,是典型的“耐旱”类型;而供应NO-3-N营养时渗透调节并未发挥明显作用,而采用典型的“避旱”类型,无法避旱时水稻生长则受到抑制。
In order to study the difference of osmotic adjustment between different forms of nitrogen (NH + 4-N and NO-3-N) and water stress interaction in rice, water stress was applied to the local roots by nutrient solution culture and PEG (6000) Changes of Main Osmotic Adjustment Substances in Various Parts of Rice. The results showed that there was no significant change in root growth on the stressed side of NH + 4-N under local root water stress, and the contents of soluble sugar and free amino acid increased by 64.5% and 167%, respectively. The content of soluble sugar in root xylem and phloem sap While the contents of soluble sugar and free amino acid increased by 243% and 42.2%, respectively. However, the content of soluble sugar in the phloem sap of roots on the stress side decreased significantly while the content of soluble sugar and free amino acid increased by 46.4% and 73.9 %, Soluble sugar content in phloem juice decreased significantly. The root biomass accumulation on the unprotected side increased significantly, which was consistent with the significant increase of soluble sugar content in the phloem sap. NH + 4-N nutrition makes rice effectively resist water stress through osmotic regulation, which is a typical “drought tolerant” type. Osmotic regulation does not play a significant role in the supply of NO-3-N nutrition, Drought-resistant "type, rice growth can be suppressed when drought can not be avoided.