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根据２，３二氨基萘（２，３ｄｉａｍｉｎｏｎａｐｈｔｈａｌｅｎｅ，ＤＡＮ）可与一氧化氮（·ＮＯ）反应生成荧光性２，３萘酚三唑（２，３ｎａｐｈｔｈｏｔｒｉａｚｏｌｅ，ＮＡＴ）和·ＮＯ的气体特性，设计了一个流动气体和荧光方法相结合的·ＮＯ直接测定方法．此法以惰性气体氮气为流动气体使样品中的待测·ＮＯ随流动气体进入测定溶液，溶液中的ＤＡＮ捕集流动气体中的·ＮＯ生成荧光性ＮＡＴ．实验结果表明测定溶液的荧光强度与·ＮＯ浓度呈正比，与酸化亚硝酸盐溶液的浓度亦呈良好线性关系．用此方法测得五份酸化正常人血浆的·ＮＯ浓度为（３１０４±４７０）ｎｍｏｌ／Ｌ，并可用于连续观察·ＮＯ从亚硝基硫醇的自发释放
According to 2,3-diaminonaphthalene (DAN), it can react with nitric oxide (NO) to generate 2,3naphthotriazole (NAT) and NO Of the gas characteristics, the design of a combination of mobile gas and fluorescence methods NO direct determination. In this method, the inert gas nitrogen is used as the flowing gas, so that the NO to be detected in the sample flows into the measuring solution with the flowing gas, and the NO in the flowing gas from the trapped DAN generates fluorescent NAT. The experimental results show that the fluorescence intensity of the determination solution is proportional to · NO concentration, and the concentration of acidified nitrite solution showed a good linear relationship. The concentration of NO in five acidified normal human plasma measured by this method was (3104 ± 470) nmol / L and was used for continuous observation. · The spontaneous release of NO from nitrosothiol