论文部分内容阅读
目的建立同时测定银菊浸膏中绿原酸、木犀草苷、3,5-O-二咖啡酰基奎宁酸含量的高效液相色谱法,优选最佳提取溶剂。方法色谱条件:Ultimate PLUS C18色谱柱(4.6 mm×250 mm,5μm),以乙腈(A)-0.1%磷酸水溶液(B)为流动相梯度洗脱,流速1.0 m L·min?1,柱温40℃,波长348 nm,比较不同提取溶剂对浸膏中3种成分的影响。结果绿原酸、木犀草苷、3,5-O-二咖啡酰基奎宁酸分别在0.077~1.920,0.082~3.288,0.141~5.624μg内线性关系良好,平均加样回收率为100.8%(RSD=2.3%),101.5%(RSD=0.7%),97.6%(RSD=0.6%);银菊浸膏乙醇提取显著优于水提取,且40%乙醇提取最佳。结论该法简便、准确,可用于银菊浸膏提取工艺优选与质量控制。
OBJECTIVE To establish a HPLC method for the simultaneous determination of chlorogenic acid, luteolin and 3,5-O-disaccharidylquinic acid in silver chrysanthemum extract, and to optimize the optimal extraction solvent. Methods The chromatographic conditions were as follows: an Ultimate PLUS C18 column (4.6 mm × 250 mm, 5 μm) eluting with a mobile phase of acetonitrile (A) -0.1% phosphoric acid (B) at a flow rate of 1.0 mL · min -1 40 ℃, the wavelength of 348 nm, compared with different extraction solvents on the three components of the extract. Results The linear relationship between chlorogenic acid, luteolin and 3,5-O-dis Caffeoylquinic acid was 0.077 ~ 1.920,0.082 ~ 3.288,0.141 ~ 5.624μg respectively with the average recovery of 100.8% (RSD = 2.3%), 101.5% (RSD = 0.7%) and 97.6% (RSD = 0.6%). The ethanol extraction of silver chrysanthemum extract was significantly better than that of water extraction, and the extraction of 40% ethanol was the best. Conclusion The method is simple and accurate, and can be used for the extraction process optimization and quality control of Yinju extract.