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目的通过优化内生放线菌WP-1的发酵条件,提高制霉色基素(funguchromin,FC)的产量。方法采用单因素试验和正交试验分析不同速效、迟效碳源,不同速效、迟效氮源以及不同无机盐对FC产量的影响,优化放线菌WP-1的培养基组分;采用单因素试验分析不同的培养基初始pH值、培养温度、接种量、装液量、摇床转速对FC产量的影响,优化放线菌WP-1的培养条件。结果优化后的发酵条件为葡萄糖1%,可溶性淀粉4%,蛋白胨0.5%,花生饼粉9%,MnSO_40.000 5%,ZnSO_4 0.000 5%,CuSO_4 0.000 2%;初始pH 5.5,发酵温度28℃,装液量50 m L培养基/250 m L三角瓶,接种量8%,转速180 r·min~(-1)。在此条件下,FC的产量可达159.0 mg·L~(-1),与初始发酵条件相比,提高了4.9倍。结论该方法优化了WP-1产生FC的发酵条件,为下一步规模发酵提供了基础数据。
Objective To improve the yield of funguchromin (FC) by optimizing the fermentation conditions of endophytic actinomycete WP-1. Methods The effects of different available and delayed carbon sources, different available and delayed nitrogen sources and different inorganic salts on the yield of FC were analyzed by single factor test and orthogonal test to optimize the culture medium components of actinomycetes WP-1. The factorial experiments were conducted to analyze the effects of initial pH value, culture temperature, inoculum size, liquid loading volume and shaking speed on FC yield, and to optimize the culture conditions of actinomycete WP-1. Results The optimal fermentation conditions were as follows: 1% glucose, 4% soluble starch, 0.5% peptone, 9% peanut meal, 5% MnSO_40.000, 5% ZnSO_4 and 0.0002% CuSO_4; initial pH 5.5 and fermentation temperature 28 ℃ , 50 mL medium / 250 mL Erlenmeyer flask, inoculation volume 8%, rotation speed 180 r · min -1. Under these conditions, the yield of FC reached 159.0 mg · L -1, 4.9 times higher than the initial fermentation conditions. Conclusion This method optimized the fermentation conditions of FC-1 produced by WP-1, and provided the basic data for the next step of scale fermentation.