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目的探讨三七总皂甙（ＰＮＳ）对颅脑损伤后脑组织中Ｃａ２＋、ＣａＭ含量的影响及其对颅脑损伤后的治疗效应。方法干湿法测定脑组织含水量，放免法、原子吸收分光光度法测定血及脑组织中Ｃａ２＋、ＣａＭ含量；分析ＰＮＳ对上述指标的影响。结果ＰＮＳ能显著降低颅脑损伤后血与脑组织中Ｃａ２＋、ＣａＭ含量，减少脑组织含水量及伊文思蓝染范围。结论ＰＮＳ能阻滞脑损伤后神经细胞内钙超载，阻断Ｃａ２＋－ＣａＭ复合物形成，减轻脑水肿和血脑屏障通透性，对颅脑损伤具有一定的脑保护作用。
Objective To investigate the effect of panax notoginseng saponins (PNS) on the content of Ca2+ and CaM in brain tissue after brain injury and its therapeutic effect on craniocerebral injury. Methods Wet and dry methods were used to measure the brain tissue water content. The levels of Ca2+ and CaM in blood and brain tissues were determined by radioimmunoassay and atomic absorption spectrophotometry. The effects of PNS on these parameters were analyzed. Results PNS can significantly reduce the content of Ca2+ and CaM in blood and brain after craniocerebral injury, and reduce the brain tissue water content and Evans blue staining range. Conclusion PNS can block the intracellular calcium overload after brain injury, block the formation of Ca2+-CaM complex, reduce cerebral edema and permeability of blood-brain barrier, and has a certain brain protection effect on brain injury.