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为从分子水平研究肠型脂肪酸结合蛋白基因(intestines fatty acid-binding protein,IFABP)在鱼类脂代谢中的作用,该研究克隆并获得了1 362 bp大黄鱼I-FABPb基因序列,采用实时荧光定量PCR技术检测了I-FABPb基因在肌肉、肝、肠、胃、性腺、鳃、脑和心脏等8个不同组织中的表达情况,研究了饥饿和复投喂对大黄鱼I-FABPb基因在肠、肌肉和肝中表达的影响。结果显示,I-FABPb基因在8个被检测组织中均有表达,但在肠中表达量最高。饥饿对大黄鱼肠、肌肉和肝中I-FABPb基因表达影响显著,均呈现了先上升后下降的趋势,但在肠中变化最显著;长期饥饿后复投喂,I-FABPb基因在肠、肌肉和肝中的表达量均显著升高(P<0.05),且显著高于饥饿0 d的水平(P<0.05)。结果表明,饥饿及复投喂明显影响大黄鱼的脂肪代谢,I-FABPb在肠道脂肪代谢中起重要作用。
In order to study the role of intestines fatty acid-binding protein (IFABP) in lipid metabolism at the molecular level, we cloned and obtained the I-FABPb gene sequence of 1 362 bp large yellow croaker Quantitative PCR was used to detect the expression of I-FABPb gene in 8 different tissues including muscle, liver, intestine, stomach, gonad, gill, brain and heart. The effects of starvation and refeeding on the expression of I- Intestinal, Muscle and Liver Expression Implications. The results showed that I-FABPb gene was expressed in all of the eight tissues tested, but the highest expression in the intestine. The effect of starvation on the expression of I-FABPb gene in intestine, muscle and liver of large yellow croaker was significant, both of which showed the trend of first increasing and then decreasing, but the most significant change in the intestine. After prolonged starvation, the I-FABPb gene was refected in intestine, Muscle and liver expression were significantly increased (P <0.05), and significantly higher than the 0 d level of hunger (P <0.05). The results showed that starvation and re-feeding significantly affect the fat metabolism of large yellow croaker, I-FABPb plays an important role in intestinal fat metabolism.