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目的 初步探讨顺铂联合环磷酰胺 (PC)化疗对卵巢癌患者外周血淋巴细胞DNA的损伤情况 ,为其临床用药方式提供理论依据。方法 采用单细胞凝胶电泳技术 (SCGE)检测 31例卵巢癌患者外周血淋巴细胞DNA损伤状况。结果 化疗前的卵巢癌患者外周血淋巴细胞DNA断裂所形成的彗星出现率及彗星样尾长与对照人群相比 ,相差无显著性 ;经联合化疗后的卵巢癌患者比化疗前及对照组的彗星出现率明显增加 ,彗星样尾长明显加长 ,分别达化疗前的 1 0 .1 9倍和 2 .2 3倍 ,相差有高度显著性 (P <0 .0 1 )。结论 顺铂联合环磷酰胺治疗卵巢癌对患者外周血淋巴细胞DNA产生一定的损伤
Objective To investigate the DNA damage of peripheral blood lymphocytes in patients with ovarian cancer treated with cisplatin combined with cyclophosphamide (PC) chemotherapy and to provide a theoretical basis for its clinical use. Methods The DNA damage status of peripheral blood lymphocytes in 31 patients with ovarian cancer was detected by single cell gel electrophoresis (SCGE). Results The incidence of comet and comet-like tail formed by DNA breakage of peripheral blood lymphocytes in patients with ovarian cancer before chemotherapy were not significantly different from those in control subjects. The ovarian cancer patients after chemotherapy were significantly lower than those before chemotherapy and in control group The incidence of comet significantly increased, and the tail length of comet was significantly longer, reaching respectively 1.19 times and 2.3 times higher than before treatment, the difference was highly significant (P <0.01). Conclusion cisplatin combined with cyclophosphamide in the treatment of ovarian cancer patients with peripheral blood lymphocytes produce some DNA damage