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背景:有关高血压与一氧化氮关系的研究主要为单一时间的对照研究,但随着血压的改变一氧化氮如何变化的研究较少。目的:观察自发性高血压大鼠(spontaneoulyhypertensiverats,SHR)血浆一氧化氮浓度的变化,探讨一氧化氮与高血压发生发展的关系,为判断高血压的预后提供理论依据。设计:非随机对照的实验研究。地点、材料和干预:实验在广州暨南大学完成。选择SHR30只,雌性6只,雄性9只(高血压组);大鼠WKY30只,雌性7只,雄性8只(对照组)。分别饲养3,6和12个月,测定血压,用硝酸银还原法测定两组大鼠血浆中一氧化氮的含量。主要观察指标:观察随着高血压的不断发展,自发性高血压大鼠血浆一氧化氮浓度的变化。结果:①血压变化:各时期对照组大鼠血压差异无显著性意义,介于(135.0±9.8)～(147.8±11.3)mmHg;高血压组大鼠血压随着月龄增加逐渐升高,12个月达(197.3±7.5)mmHg,各时间点均明显高于对照组大鼠(t=4.132～7.921,P<0.01)。②血浆一氧化氮浓度的变化:对照组大鼠血浆一氧化氮浓度各时间点差异无显著性意义(P>0.05),维持在(11.5±3.9)～(14.4±3.4)μmol/L水平;高血压组大鼠血浆一氧化氮浓度在3,6个月分别为(16.6±4.5)和(18.5±4.2)μmol/L,明显高于对照组大鼠,但在12个月却明显较对照组大鼠低犤(9.3±3.6)μmol/
BACKGROUND: Studies on the relationship between hypertension and nitric oxide are mainly single-time controlled studies. However, there is little research on how nitric oxide changes with the change of blood pressure. Objective: To observe the changes of plasma nitric oxide concentration in spontaneoulyhypertensive rats (SHR) and to explore the relationship between nitric oxide and the development of hypertension and to provide a theoretical basis for judging the prognosis of hypertension. Design: Non-randomized controlled experimental study. Location, Materials and Interventions: The experiment was performed at Guangzhou Jinan University. A total of 30 SHRs, 6 females, 9 males (hypertension group), 30 WKY rats, 7 females and 8 males (control group) were selected. The rats were respectively fed for 3, 6, and 12 months. Blood pressure was measured and the content of nitric oxide in the plasma was measured by silver nitrate reduction method. MAIN OUTCOME MEASURES: To observe the changes of plasma nitric oxide concentration in spontaneously hypertensive rats with the continuous development of hypertension. Results: ① The changes of blood pressure: There was no significant difference in the blood pressure between the control group and the control group (135.0 ± 9.8) ~ (147.8 ± 11.3) mmHg at each stage. The blood pressure in the hypertension group increased gradually with age (197.3 ± 7.5) mmHg in each month, which were significantly higher than those in the control group at each time point (t = 4.132-7.921, P <0.01). (2) The changes of plasma nitric oxide concentration: There was no significant difference in plasma nitric oxide concentration in the control group at various time points (P> 0.05), and maintained at the level of (11.5 ± 3.9) ~ (14.4 ± 3.4) μmol / Plasma nitric oxide levels in hypertensive rats at 3 and 6 months were (16.6 ± 4.5) and (18.5 ± 4.2) μmol / L, respectively, which were significantly higher than those in control rats, but significantly higher at 12 months Rats in group B (9.3 ± 3.6) μmol /