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目的以银杏叶对照提取物为对照建立银杏叶滴丸中萜类内酯的定量测定方法。方法采用HPLC-ELSD法,色谱柱为Diamonsil C18柱(150 mm×4.6 mm,5μm),流动相为甲醇-水(30∶70),体积流量0.8 mL/min,ELSD检测器,漂移管温度105℃,氮气体积流量3.0 L/min。结果银杏内酯A、银杏内酯B、银杏内酯C、白果内酯分别在1 672.2～10 033.2、1 026.4～6 158.4、527.8～3 166.8、2 200.8～13 204.8 ng呈良好的对数线性关系;银杏内酯A、银杏内酯B、银杏内酯C、白果内酯及总萜类内酯的平均回收率分别为100.3%(RSD 2.5%)、108.1%(RSD 3.5%)、116.0%(RSD 4.9%)、90.4%(RSD 0.6%)及99.3%(RSD 1.1%)。结论本方法简单易行、可控性强、重复性好,可用于银杏叶滴丸中萜类内酯的定量测定,并为中成药中多指标成分同时测定提供了有效的思路和解决方案。
Objective To establish a method for the quantitative determination of terpene lactones in Ginkgo biloba pills by using Ginkgo biloba extract as control. Methods The HPLC-ELSD method was used on a Diamonsil C18 column (150 mm × 4.6 mm, 5 μm) with a mobile phase of methanol-water (30:70), a volume flow of 0.8 mL / min, an ELSD detector ℃, nitrogen volume flow 3.0 L / min. Results Ginkgolide A, ginkgolide B, ginkgolide C and bilobalide showed good logarithmic linearity at 1 672.2 ~ 10 033.2, 1 026.4 ~ 6 158.4, 527.8 ~ 3 166.8 and 2 200.8 ~ 13 204.8 ng respectively The average recoveries of ginkgolide A, ginkgolide B, ginkgolide C, bilobalide and total terpene lactones were 100.3% (RSD 2.5%), 108.1% (RSD 3.5%), 116.0% (RSD 4.9%), 90.4% (RSD 0.6%) and 99.3% (RSD 1.1%). Conclusion The method is simple and easy to operate with strong controllability and repeatability. It can be used for the quantitative determination of terpene lactones in Ginkgo biloba dropping pills and provides an effective method and solution for the simultaneous determination of multi-index components in Ginkgo biloba.