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目的了解新疆昌吉部分地区野生动物自然感染恙虫病东方体(Ot)的情况。方法采用多种方法捕鼠类,提取DNA,应用巢式PCR检测Ot-Sta56基因;阳性核苷酸序列片段测序,通过美国国家生物技术信息中心网站对基因序列进行BLAST比较分析。结果共捕获鼠432只、鸟类53只,仅在木垒县的鼠类、鸟类脾样本中检测到阳性片段,感染率分别为5.3%、11.1%;其他县市未检测到阳性。DNA序列分析表明,鼠类、鸟类阳性标本中扩增产物的核苷酸序列相同,碱基长度均为442bp。BLAST表明目的基因与Karp型的碱基序列同源性最高(98.5%),应属于Karp型。结论新疆昌吉地区木垒县啮齿类及鸟类动物中可能存在Ot感染。
Objective To understand the natural infection of Otomycosis orientalis (Ot) by wild animals in some parts of Changji, Xinjiang. Methods A large number of methods were used to catch mice and DNA was extracted. The nested PCR was used to detect Ot-Sta56 gene. The positive nucleotide sequence was sequenced and BLAST was used to analyze the gene sequence by the National Center for Biotechnology Information. Results A total of 432 rats and 53 birds were captured. Positives were detected only in murine and spleen samples of Mulei County, with infection rates of 5.3% and 11.1%, respectively. No positive results were detected in other counties. DNA sequence analysis showed that the nucleotide sequences of the amplified products in the positive samples of mice and birds are the same, and the lengths of the bases are all 442 bp. BLAST showed that the homology of the target gene with Karp type was the highest (98.5%), which belongs to Karp type. Conclusion There may be Ot infection in rodents and birds in Mule County, Changji of Xinjiang.