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目的 观察一氧化氮 (nitricoxide ,NO)对人鼻粘膜纤毛运动的影响。方法 建立慢性鼻窦炎鼻息肉粘膜上皮细胞原代培养模型 ,用电视显微镜法观察NO前体L 精氨酸对纤毛运动的影响 ,统计学方法采用配对t检验。结果 L 精氨酸能使纤毛运动频率由加药前的每分钟 (4 0 0± 48)次增加到加药后的每分钟 (793± 6 8)次 (n =9,P <0 .0 0 1) ;其同分异构体D 精氨酸无此作用 ;一氧化氮合酶(nitricoxidesynthase,NOS)抑制剂硝基 L 精氨酸甲酯能明显抑制L 精氨酸的作用。结论 原代培养慢性鼻窦炎鼻息肉粘膜上皮细胞能合成NOS ,L 精氨酸在NOS的作用下产生NO ,从而加快纤毛细胞的纤毛运动。
Objective To observe the effect of nitric oxide (NO) on the movement of human nasal mucociliary. Methods The primary culture model of mucosal epithelial cells of chronic sinusitis and nasal polyps was established. The effect of L-arginine (NO) precursor on ciliary motility was observed by TV microscope, and the paired t-test was used for statistical analysis. Results L-arginine could increase the frequency of ciliary movement from 400 ± 4 minutes per minute before drug administration to 793 ± 6 8 minutes per minute after drug administration (n = 9, P <0. 0 0 1). The isomer D arginine had no such effect. Nitric oxide synthase (L-arginine methyl ester), a nitric oxide synthase (NOS) inhibitor, significantly inhibited L-arginine. Conclusion Primary culture of chronic sinusitis and nasal polyps mucosal epithelial cells can synthesize NOS, L arginine produce NO under the action of NOS, thereby accelerating ciliary cell cilia movement.