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目的观察禁欲时间对男性不育症患者精子核蛋白组型转换半定量检测值的影响。方法对2005年11月至2007年11月期间来常州市中医医院男科门诊就诊的452例不育症患者,采用苯胺蓝染色法进行精子核蛋白组型转换半定量的检测。结果完成的452例患者中,核蛋白组型转换半定量检测值正常者362例(80.09%),异常者90例(19.91%)。按照禁欲时间分为小于3d组、3～7d组与大于7d组进行比较,核蛋白组型转换半定量检测值差异有统计学意义(P<0.05)。结论精子核蛋白组型半定量的检测对男性不育症有临床价值,禁欲时间对核蛋白组型转换半定量检测值有一定影响。
Objective To observe the influence of abstinence time on semi-quantitative detection of sperm nucleoprotein conversion in male infertility patients. Methods From November 2005 to November 2007, 452 cases of infertility were treated by male outpatient department of Changzhou TCM Hospital, and the semiquantitative detection of sperm nucleoprotein conversion was performed by aniline blue staining. RESULTS: Of the 452 patients completed, 362 (80.09%) had normal semiquantitative detection of nucleoprotein conversion and 90 (19.91%) had abnormalities. According to the time of abstinence is divided into less than 3d group, 3 ~ 7d group and more than 7d group for comparison, semi-quantitative detection of nucleoprotein conversion type difference was statistically significant (P <0.05). Conclusion The semi-quantitative detection of sperm nucleoprotein type has clinical value in male infertility, and the time of abstinence has a certain influence on semi-quantitative detection of nucleoprotein conversion.