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目的探讨普通轮状病毒(RV)腹泻患儿病毒血症出现情况。方法套式-逆转录PCR检测血中RV,阳性PCR产物测序。结果 60例RV腹泻患儿中,检出1例病毒血症阳性者,检出率为1.67％,测序证实为RV序列,未见明显变异。结论普通RV腹泻患儿也可发生病毒血症。
Objective To investigate the occurrence of viremia in children with ordinary rotavirus (RV) diarrhea. Methods Nested reverse transcription polymerase chain reaction (RT - PCR) was used to detect RV in blood, and the positive PCR products were sequenced. Results Among the 60 children with RV diarrhea, one case of positive viremia was detected, with a detection rate of 1.67%. Sequencing was confirmed as RV sequence with no obvious variation. Conclusion Children with RV may also develop viremia.