论文部分内容阅读
作者用DNA原位定量的方法,研究了5-羟色胺(5-HT)对受~(60)Coγ线8Gy全身照射后第2日人鼠小肠腺细胞DNA的辐射防护作用。发现,照射前给5—HT防护组小肠腺细胞DNA含量显著低于正常对照组(P<0.05),但又非常显著地高于单纯照射组(P<0.01).正常小肠腺DNA直方图是二倍体(2c)直方图,峰值位于2c处.5—HT防护组小肠腺细胞DNA直方图的峰值左移到靠近一倍体(1c)处,但仍保留约20％的2c～3c的小肠腺细胞。单纯照射组DNA直方图的峰值也左移到靠近1c处.却完全缺乏2c以上的小肠腺细胞。以上结果提示,5—HT对小肠腺细胞DNA有辐射防护作用。
Using DNA in situ quantification, the authors studied the protective effect of serotonin (5-HT) on the DNA of small intestine gland cells of human mice on day 2 after 8Gy irradiation with 60Co γ line. It was found that the DNA content of small intestine gland cells in the 5-HT protective group before irradiation was significantly lower than that in the normal control group (P<0.05), but it was significantly higher than that in the simple irradiation group (P<0.01). The normal small intestine gland DNA histogram was two. Ploidy (2c) histogram, peak at 2c. 5-HT protection group Small peaks of small intestine gland cell DNA histogram left to near the diploid (1c), but still retain about 20% of 2c to 3c small intestine gland cells . The peak of the histogram of DNA in the simple irradiation group also moved to the left near 1c, but the small intestine gland cells were completely lacking. The above results suggest that 5-HT has radioprotective effect on DNA of small intestine gland cells.