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在26S泛素蛋白酶体途径介导的蛋白质降解过程中,RING finger蛋白是一类特殊的泛素蛋白连接酶,参与植物多个生长发育及逆境胁迫响应过程。为探明柚RING finger家族成员的序列特征及表达模式,从红肉蜜柚和琯溪蜜柚果实汁胞中分离出两个RING finger基因cDNA片段(CgRHF1和CgRHF2),采用实时荧光定量PCR研究其在不同组织及果实发育不同时期的表达模式。结果表明,两个基因均属于RING-H2 finger家族成员,且红肉蜜柚与琯溪蜜柚的两个CgRHF之间碱基序列均无差异;除根组织外,CgRHF1表达水平较CgRHF2高,且在红肉蜜柚茎、叶和花中的表达水平显著高于琯溪蜜柚;CgRHF2在组织及果实发育过程表达水平均较低,但在果实成熟采摘阶段急剧上升。CgRHF1与CgRHF2表达模式的差异暗示这两个基因在柚组织及果实发育进程中行使不同的生物学功能,而非功能互补基因。
RING finger protein is a kind of special ubiquitin protein ligase which participates in the process of plant multiple growth and stress responses during the process of protein degradation mediated by 26S ubiquitin proteasome pathway. To identify the sequence characteristics and expression patterns of RING finger family members, two RING finger cDNA fragments (CgRHF1 and CgRHF2) were isolated from the juice of Pomelo and Citrus pummelo fruit juice. Real-time fluorescence quantitative PCR Its expression patterns in different tissues and fruit development at different stages. The results showed that the two genes belong to the RING-H2 finger family, and there was no difference in the nucleotide sequence between the two CgRHFs of the red meat pomelo and the pomfret. The expression level of CgRHF1 was higher than that of CgRHF2 The expression level of CgRHF2 in stem, leaf and flower of red meat pomelo was significantly higher than that in Guanxi honey pomelo. The expression level of CgRHF2 in tissue and fruit development was lower, but rose sharply in the mature fruit picking stage. The difference of CgRHF1 and CgRHF2 expression patterns implied that these two genes exert different biological functions in pomelo and fruit development, but not function complementary genes.