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本文应用免疫组化技术显示喉重度发育异常和原位癌的表皮生长因子受体(epidermal growth factor receptor,EGFR),旨在确立EGFR是否存在于这些癌前损伤,以便今后在不甚明显的发育异常中识别这类损伤。研究对象是14例病理已明确为重度发育异常和原位癌的活检标本,标本切成5μm厚切片,去石蜡后组织片先用3%过氧化氢覆盖10分钟,进而与鼠抗EGFR的单克隆抗体孵育60分钟;然后与生物素基处理的山羊抗鼠免疫球蛋白孵育20分钟,再与用链霉抗生物素蛋白标记的过氧化物酶孵育20分钟,过氧化物酶用含0.03%过氧化氢的3,3′,二氨基联苯胺四氯化物显影。组织片最后经Mayer′s苏木精和0.2%氧化铵重复染色并脱水后观察。全部孵育程序均在室温下进
Here we use immunohistochemistry to show the occurrence of laryngeal dysplasia and carcinoma in situ of epidermal growth factor receptor (EGFR) in order to establish whether EGFR is present in these pre-cancerous lesions so that in the future with less pronounced development Abnormalities identify such damage. The study was performed on 14 biopsy specimens with pathologically confirmed severe dysplasia and carcinoma in situ. The specimens were cut into 5-μm-thick sections. The paraffin-embedded tissue sections were first covered with 3% hydrogen peroxide for 10 minutes and then incubated with a single anti-EGFR The cloned antibody was incubated for 60 minutes; then incubated with biotin-treated goat anti-mouse immunoglobulin for 20 minutes and incubated with streptavidin-labeled peroxidase for 20 minutes. Peroxidase was incubated with 0.03% Hydrogen peroxide 3,3 ', diaminobenzidine tetrachloride development. Tissue pieces were finally stained repeatedly with Mayer's hematoxylin and 0.2% ammonium oxide and observed after dehydration. All incubations are performed at room temperature