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[目的]建立新生儿脐带血中多氯联苯(PCBs)的分析方法,并对电子垃圾拆解区新生儿PCBs暴露水平进行评估。[方法]血清样品经正己烷和甲基叔丁基醚混合溶液(1:1)萃取,经干燥得到脂肪并称取其质量。脂肪经正己烷溶解、硫酸净化、酸性硅胶柱净化及浓缩定容后,利用气相色谱-质谱联用仪检测分析PCBs含量。采用EI源电离方式与选择离子检测法(SIM),以外标法进行定量。[结果]加标回收率76.0%～113.0%;方法检出限0.01～0.12ng/g;电子垃圾拆解区新生儿体内28种PCBs的总浓度为338.56 ng/g lipid。[结论该方法检测新生儿脐带血中28种PCBs的同系物,灵敏度高、重复性好、回收率良好,可用于相关样品的检测。广东省汕头贵屿镇电子垃圾拆解区新生儿PCBs已处于较高暴露水平,值得重视。
[Objective] To establish a method for the analysis of polychlorinated biphenyls (PCBs) in neonatal umbilical cord blood and to evaluate the PCBs exposure of newborns in the dismantling area of electronic rubbish. [Method] The serum samples were extracted with a mixture of n-hexane and methyl tert-butyl ether (1: 1), dried to obtain fat and weighed. Fat was dissolved in n-hexane, sulfuric acid purification, acidic silica gel column purification and concentration, using gas chromatography-mass spectrometry detection and analysis of PCBs content. EI source ionization method and selective ion detection (SIM) were used to quantify by external standard method. [Results] The spiked recoveries ranged from 76.0% to 113.0%. The detection limits were from 0.01 to 0.12 ng / g. The total concentration of 28 PCBs in neonates with e-waste disposal area was 338.56 ng / g lipid. [Conclusion] This method detects the homologues of 28 kinds of PCBs in cord blood of newborn with high sensitivity, good repeatability and good recovery and can be used for the detection of related samples. The newborn PCBs in the e-waste dismantling area of Guiyu town, Shantou, Guangdong province are at a high level of exposure and deserve attention.