,Tumor suppressor miR-128-3p inhibits metastasis and epithelial-mesenchymal transition by targeting

• 作 者: Lili Zhao Rui Li Shanling Xu Yi Li Pei Zhao Wei Dong Zhenjun Liu Qian Zhao Bo Tan
• 机 构: Intensive Care Unit, Sichuan Cancer Hospital & Institute, Sichuan Cancer Center, Chengdu 610000, Chi
• 出 处: 生物化学与生物物理学报（英文版）
• 发表日期: 2018年2期
• 关键词: miR-128-3p esophageal squamous-cell carcinoma ZEB1 epithelial-mesenchymal transi

MicroRNAs (miRNAs) are some short RNAs that regulate multiple biological functions at post-transcriptional levels,such as tumorigenic processes,inflammatory lesions and cell apoptosis.Zinc finger E-box binding homeobox factor 1 (ZEB1) is a crucial mediator of epithelial-mesenchymal transition (EMT).It induces malignant progression of various cancers including human esophageal squamous-cell carcinoma (ESCC).In this study,we found that miR-128-3p was down-regulated in ESCC tissues and cells by using PCR.Moreover,down-regulated expression of miR-128-3p was testified to be associated with poor prognosis of ESCC patients and might be regarded as an independent prognostic factor.Then,we examined the role of miR-128-3p in ESCC cells,and found that miR-128-3p could suppress the cell migration and invasion in vitro.Furthermore,ZEB1 was confirmed to be a direct target of miR-128-3p by luciferase reporter assay.Rescue experiments proved that EMT was regulated by miR-128-3p via suppression of ZEB1.Taken all together,we conclude that miR-128-3p suppresses EMT and metastasis via ZEB1,and miR-128-3p may be a critical mediator in ESCC.