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作者研究了曼氏血吸虫全虫匀浆(WWH)和成虫表膜抗原(mb-S)在小鼠引起的免疫应答,佐剂和皂素介导的保护性免疫及免疫接种后某些抗体应答与所获得保护性的关系。曼氏血吸虫成虫于聚四氟乙烯组织研磨器中制备成悬液,经10000g离心2分钟,吸取上清,即为WWH。取WWH于4℃100000g离心1小时,收集上清。其沉淀重新悬浮于PBS。根据Simpon等(1981)的方法制备mb-S,仓鼠感染6周后收集成虫,
The authors studied the immune response caused by Schistosoma mansoni homogenate (WWH) and adult membrane antigen (mb-S) in mice, protective immunity mediated by adjuvants and saponin, and some antibody responses following immunization Relationship with the acquired protection. Schistosoma mansoni adults Teflon tissue grinder prepared as a suspension, after centrifugation at 10000g for 2 minutes, the supernatant, that is WWH. Take WWH at 4 ℃ 100000g centrifugal 1 hour, the supernatant was collected. The pellet was resuspended in PBS. Mb-S was prepared according to the method of Simpon et al. (1981), and adults were harvested after 6 weeks of infection by hamsters,