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目的重组腺病毒反义ＩＧＦ－１基因（ＡｄＨＣＭＶ－ＩＧＦ－１Ａ）治疗大鼠Ｃ６胶质瘤。方法体外克隆反义ＩＧＦ－１３００ｂＰ基因片段重组入腺病毒，经扩增纯化，测定滴度，体外体内感染Ｃ６胶质瘤细胞，观察ＩＧＦ－１的表达及肿瘤大小、生存期变化。结果重组腺病毒反义ＩＧＦ－１滴度６．５×１０１０ｐｆｕ／ｍｌ，反义ＩＧＦ－１基因序列正确，体外Ｃ６－Ａｄ－ＩＧＦ－１Ａ的ＩＧＦ－１表达降低，Ａｄ－ＩＣＦ－１Ａ治疗皮下Ｃ６肿瘤细胞接种组，肿瘤完全消失。与对照组相比有显著性差异（Ｐ＜０．０１），Ａｄ－ＩＧＦ－１Ａ治疗脑内Ｃ６肿瘤细胞接种组，大鼠生存期超过９０天，而对照组分别为１６．５±１．４天（Ａｄ－ｌａｃｚ组）、１５．７±１．６天（生理盐水组），Ｐ＜０．００１。结论重组腺病毒反义ＩＧＦ－１可抑制Ｃ６细胞ＩＧＦ－１的表达，ＡｄＨＣＭＶ－ＩＧＦ－１Ａ治疗大鼠Ｃ６胶质瘤效果显著。
Objective To investigate the effect of adenovirus antisense IGF-1 gene (AdHCMV-IGF-1A) on C6 glioma in rats. METHODS: Antisense IGF-1300bP gene fragment was cloned into adenovirus in vitro. After amplification and purification, titer was measured and C6 glioma cells were infected in vitro. The expression of IGF-1, tumor size and survival time were observed. Results Antisense IGF-1 titer of recombinant adenovirus was 6.5×1010 pfu/ml. Antisense IGF-1 gene sequence was correct. IGF-1 expression of C6-Ad-IGF-1A was decreased in vitro. Ad-ICF-1A treatment was performed subcutaneously. In the C6 tumor cell inoculation group, the tumor completely disappeared. Compared with the control group, there was a significant difference (P <0.01), Ad-IGF-1A treatment of intracerebral C6 tumor cell inoculation group, the survival time of rats over 90 days, while the control group was 16.5 ± 1. 4 days (Ad-lacz group), 15.7±1.6 days (saline group), P<0.001. Conclusion The recombinant adenovirus antisense IGF-1 can inhibit the expression of IGF-1 in C6 cells, and AdHCMV-IGF-1A has a significant effect on C6 glioma in rats.