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对转AmGS基因(从沙冬青中克隆的肌醇半乳糖苷合成酶基因)的红叶石楠植株进行多项分子检测(PCR,Southern,RT-PCR),结果表明AmGS基因已经整合到转基因株系R6和R7的基因组DNA中,并检测到转录水平上的表达。随后,经对R6和R7两个转基因株系进行连续6代芽切扩繁继代株系的PCR检测,发现导入的AmGS基因传递到所有芽切扩繁后代植株中。植株抗寒能力试验结果表明,在相同低温处理条件下,转基因株系均比未转基因植株的存活率要高。相对电导率测定结果表明,随着处理温度的降低,2个转基因株系相对电导率升高的程度明显低于未转基因的对照植株。低温半致死温度(LT50)测定结果表明,2个转基因株系(R6和R7)的LT50明显低于未转基因的对照植株。上述结果说明导入的AmGS基因提高了转基因株系的抗寒性。
A number of molecular tests (PCR, Southern, RT-PCR) of the AmGS gene (the galactinol synthase gene cloned from A. asarum) were carried out. The results show that the AmGS gene has been integrated into the transgenic line R6 And R7 genomic DNA, and expression at the transcriptional level was detected. Subsequently, PCR analysis of R6 and R7 transgenic lines for 6 consecutive generations of buds and propagation of the progeny lines revealed that the introduced AmGS gene was transferred to all progeny shoots of the progeny. Cold-tolerant plants test results show that the same low-temperature treatment conditions, transgenic lines than non-transgenic plants have a higher survival rate. Relative conductivity measurements showed that the relative electrical conductivity of the two transgenic lines was significantly lower than that of the untransformed control plants as the treatment temperature was decreased. LT50 results showed that the LT50 of two transgenic lines (R6 and R7) was significantly lower than that of the non-transgenic control plants. The above results indicate that the introduced AmGS gene enhances the cold resistance of the transgenic lines.