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本研究在巴布亚新几内亚东Sepik省的Dreikikir区的5个村进行。自1993年4月至1994年3月(10月除外),每月进行4次,从傍晚6时至次晨6时在室内及室外捕捉叮停在志愿者足及小腿部的蚊虫。根据形态学,以刻点按蚊复合体所独具的扇形斑作为区分它们与其它蚊种的标志。鉴定雌蚊蚊种,贮存在70％甲醇中转至实验室。经梅氏苏木素染色后解剖,镜检头、胸、腹三部的幼丝虫。 根据公式计算年叮咬率(ABR),年感染性叮咬率(AIBR)及年传播潜能(ATP)。首先计算出月传播指标,月叮咬率(MBR)是在1个月内从傍晚6时至次晨6时叮咬1个人的估计雌蚊数;而月感染性叮咬率(MIBR)是月叮咬率乘以L_3蚊的百分比;月传播潜能
The study was conducted in five villages in Dreikikir district, East Sepik province, Papua New Guinea. From April 1993 to March 1994 (except October), four times a month, and from 6 pm to 6 am, indoor and outdoor mosquitoes were dredged in volunteer feet and calves. According to morphology, the scalloped fan-shaped spots of the Anopheles complex are used as markers to distinguish them from other mosquito species. Female mosquitoes were identified and stored in 70% methanol and transferred to the laboratory. Mehshematoxylin staining after dissection, microscopic examination head, thoracic, abdomen tripartite young filariasis. Annual bite rate (ABR), annual infectious bite (AIBR) and annual transmission potential (ATP) were calculated according to the formula. First, the monthly transmission index is calculated. The monthly bite rate (MBR) is the estimated number of female mosquitoes that bite one person from 6:00 a.m. to 6:00 a.m. in one month. The monthly bite-in-touch rate (MIBR) Multiplied by the percentage of L_3 mosquitoes; monthly transmission potential